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SKYCAM Confocal Microscopy

SKYCAM Confocal Microscopy




  • Leica TCS SP8 Laser Scanning Confocal Microscope with four laser lines and associated optics. The microscope was acquired through a Major Research Instrumentation Grant funded by the National Science Foundation in 2019 with Dr. Srivastava as the PI.


Capabilities of Microscope


  • The microscope can perform routine scanning, Z-Stacking, Unmixing of spectral overlap, post-acquisition image processing.
    • Available Laser Lines: 405 nm, 488 nm, 552 nm, 638 nm.
    • Available Objectives: 10X, 20X Immersion, 40X Water Immersion, 63X Oil. All objectives Plan Apo.


Technical Description


  • DM6 CS 5 pos fluo
  • Optical Outfit + ICT
  • SolaSEII 365nm
  • Transmitted Light Brightfield Detector
  • Scanning stage DM6 CS
  • Scan optics module HIVIS with rotation
  • FOV scanner SP8
  • SP8 LIAchroics Compact RGB
  • Four Internal Detector Channels pos. 1, 2, 3, 4
  • Internal Detector Channel PMT 1
  • Internal Detector Channel HyD 2
  • Internal Detector Channel PMT 3
  • Internal Detector Channel HyD 4
  • Laser 405 nm DMOD Compact
  • Laser 488 nm, 552 nm, 638 nm
  • Confocal Super-Resolution LAS X LIGHTNING
  • Premium CUDA Workstation, Monitor
  • Computer table small, 80x80 cm
  • Confocal module with patented filter-free spectral Leica SP detector for up to five individually regulatable channels.
  • Highly efficient spectral separation by unique prism design.
  • Equal brightness between channels due to W-shaped slit design. 
  • Leica LIAchroics are low incident angle dichroic beam splitters custom designed by Leica Microsystems inhouse.
  • The LIAchroic approach allows steeper cutoff and higher transmission than other dichroic beam splitter designs resulting in high contrast images.
  • Scan optics with HIVIS coating for maximal transmission from 400-800 nm.


  • The LIGHTNING image information extraction package serves for maximum extraction of image details and for confocal maximum resolution, thus expanding the imaging portfolio both in the classical range and beyond the diffraction limit. Even with an extremely low signal-to-noise ratio, LIGHTNING enables structures to be extracted from data sets that would otherwise not be visible or accessible. On the other hand, structures up to 120nm in several colors can be resolved simultaneously and at high recording speeds.


Service Fees


  • WKU Users: $25/hour
  • External Users: $40/hour


Please contact Dr. Ajay Srivastava (ajay.srivastava@wku.edu) for more details.



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 Last Modified 1/8/24